| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 36 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Phospho-Pim-1 (Tyr309) Rabbit Polyclonal Antibody detects endogenous levels of Pim-1 protein only when phosphorylated at Tyr309. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Pim-1 is a serine/threonine kinase that plays a crucial role in cell signaling pathways, particularly in lymphoid cell transformation, where Pim-1 cooperates with c-Myc. This interaction is vital for the regulation of cell proliferation and survival, making Pim-1 an important factor in oncogenesis. Pim-1 expression increases during the transition from early to late G1 phase of the cell cycle, remaining elevated at the G1/S boundary and throughout the G2 phase, which underscores its significance in cell cycle regulation. Pim-1 is regulated at both transcriptional and translational levels, with Pim-1 expression being induced by interleukin-2 stimulation, highlighting Pim-1′s role in T cell differentiation and function. |
| Product Image Gallery | |
 Western blot analysis of extracts on HuvEc cells treated with PMA, using Phospho-Pim-1 (Tyr309) Rabbit Polyclonal Antibody (EAB11397) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.
Predicted band size: 36 kDa; Observed band size: 45 kDa |
