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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 30 | Human, Mouse |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 | |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | TIGAR Rabbit Polyclonal Antibody detects endogenous levels of TIGAR protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
TIGAR also known as C12orf5 is regulated as part of the p53 tumor suppressor pathway, which is vital for maintaining genomic stability and preventing tumorigenesis. TIGAR functions by modulating the glycolytic pathway; TIGAR shares sequence similarity with the fructose-2,6-bisphosphate degrading enzyme, allowing TIGAR to lower intracellular levels of fructose-2,6-bisphosphate. This action effectively inhibits glycolysis and redirects metabolic flux towards the pentose phosphate pathway, which is essential for generating NADPH and ribose-5-phosphate. By decreasing the concentration of reactive oxygen species, TIGAR helps protect cells from oxidative stress, which can lead to DNA damage and apoptosis. |
| Product Image Gallery | |
 Western blot analysis of extracts on 5-8F, KB cells, using TIGAR Rabbit Polyclonal Antibody (EAB11404) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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