| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IP, ELISA | 152 | Human, Mouse |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IP=1:20-200 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Brd4 Rabbit Polyclonal Antibody detects endogenous levels of Brd4 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Brd4 is a member of the bromodomain and extraterminal (BET) family, characterized by two bromodomains that facilitate interaction with acetylated lysines on histones and non-histone proteins. This interaction regulates gene expression, particularly in cell cycle progression and transcriptional activation. Brd4 plays a significant role in assembling transcriptional machinery at target gene promoters, essential for proper cell growth and proliferation. Dysregulation of Brd4 has been implicated in various cancers, where aberrant function can lead to uncontrolled cell division and tumor progression. Understanding Brd4′s role in cellular processes highlights both normal physiological importance and potential as a therapeutic target in cancer treatment. |
| Product Image Gallery | |
 Western blot analysis of extracts on 293, K562 cells, using Brd4 Rabbit Polyclonal Antibody (EAB11446) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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