| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, IHC-P, IF/ICC, ELISA | 47 | Human, Mouse, Rat |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:50-300 IF/ICC=1:50-300 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | ACTL6/BAF53 Rabbit Polyclonal Antibody detects endogenous levels of ACTL6/BAF53 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
BAF53 also known as ACTL6 is a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. BAF53 plays a significant role in regulating gene expression by facilitating ATP-dependent alterations in chromatin structure. BAF53 is primarily located in both the nucleus and cytoplasm, which is vital for shuttling between these compartments and participating in various cellular processes. BAF53′s interaction with other proteins, such as TIP49 and TIP48, is essential for mediating c-Myc oncogenic activity, highlighting BAF53′s importance in cellular growth and proliferation. Additionally, BAF53 contains a conserved N-terminal motif with specific residues at serine 2 and tyrosine 6 that are critical for activity, underscoring the intricate regulatory mechanisms that govern BAF53′s function in chromatin remodeling and gene expression. |
| Product Image Gallery | |
 Western blot analysis of extracts on COLO205, Jurkat cells, using ACTL6/BAF53 Rabbit Polyclonal Antibody (EAB11464) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
 Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue, using ACTL6/BAF53 Rabbit Polyclonal Antibody (EAB11464) at 1:100 dilution.
 Immunofluorescence analysis of HuvEc cell, using ACTL6/BAF53 Rabbit Polyclonal Antibody (EAB11464) at 1:100 dilution.
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