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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB | 103 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 | |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | MGEA5 Rabbit Polyclonal Antibody detects endogenous levels of MGEA5 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
MGEA5 also known as NCOAT, HEXC, MEA5, OGA is a bifunctional enzyme that functions as both a β-hexosaminidase and a histone acetyltransferase. The enzymatic activity of NCOAT is optimal at a slightly acidic pH of 5.7-7 and NCOAT function is competitively inhibited by free N-acetylglucosamine. Due to alternative splicing events, NCOAT is expressed as three isoforms. Isoform 1 localizes to the cytoplasm, while isoform 3 localizes to the nucleus. Expressed ubiquitously with highest expression in placenta, brain and pancreas, NCOAT functions as a glycosidase that catalyzes the cleavage of O-GlcNAc residues from GlcNAc-modified proteins. In addition, NCOAT acetylates specific residues on Histone H3 and Histone H4, suggesting an important role in the histone code. |
| Product Image Gallery | |
 Western blot analysis of extracts on HepG2 cells, using MGEA5 Rabbit Polyclonal Antibody (EAB11477) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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