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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IP, IF/ICC | N/A | N/A |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:5000-20000 IP=1:100-500 IF/ICC=1:500-2000 |
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| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | His-Tag Mouse Monoclonal Antibody detects endogenous levels of His-Tag protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | N/A |
| Entrez Gene ID | N/A |
| Product Description | |
His-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells. |
| Product Image Gallery | |
 Western blot of HA-Tag fusion protein with His-Tag Mouse Monoclonal Antibody (EAB11287) at 1:5000 and 1:10000 dilution.
 Immunofluorescence analysis of 293 cell transfected with a His-Tag protein, using His-Tag Mouse Monoclonal Antibody (EAB11287) at 1:1000 dilution.
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