www.ebiocell.com
| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG2b | Monoclonal | WB, ELISA | 177 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:1000-5000 FC=1:50-200 |
|
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Nestin Mouse Monoclonal Antibody detects endogenous levels of Nestin protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Nestin is a crucial intermediate filament protein predominantly found in embryonic central nervous system progenitor cells, where it plays a vital role in maintaining the structural integrity and function of these cells during development. Its dynamic presence is also significant in muscle development, as it polymerizes with other intermediate filament proteins like Desmin and Vimentin, forming a robust network essential for cellular architecture. Nestin′s ability to co-assemble with Vimentin or α-internexin into heterodimer coiled-coil structures further contributes to the formation of 10 nm intermediate filaments, which are critical for cellular resilience. |
| Product Image Gallery | |
 Western blot analysis of extracts on NEK293, PC12 cells, using Nestin Mouse Monoclonal Antibody (EAB21167) at 1:5000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
|
