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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP, IHC-P, IF/ICC | 72 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IP=1:10-100 IHC-P=1:50-200 IF/ICC=1:50-200 |
|
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | GRP78/BiP Rabbit Monoclonal Antibody detects endogenous levels of GRP78/BiP protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
GRP78(Glucose regulated protein 78) also known as BiP, HSPA5 is a member of the heat shock protein 70 (HSP70) family, primarily localized in the endoplasmic reticulum (ER) of cells. GRP78′s presence in the ER is vital as a molecular chaperone, ensuring correct folding and assembly of nascent proteins while maintaining protein homeostasis. This function is essential for preventing misfolded protein accumulation, which can lead to ER stress and activate the unfolded protein response (UPR), a critical cellular stress response associated with various diseases, including cancer and neurodegenerative disorders. GRP78 interacts with several specific proteins, including ATF6, IRE1, and PERK, which are key players in the UPR pathway. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, HepG2, Raw264.7, C6 cells, using GRP78/BiP Rabbit Monoclonal Antibody (EAB22370) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 72 kDa; Observed band size: 78 kDa |
