| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG2a | Monoclonal | WB, IHC-P, FC, ELISA | 34 | Human, Mouse |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:100-500 FC=1:50-200 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | CDK4 Mouse Monoclonal Antibody detects endogenous levels of CDK4 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
CDK4 is a vital member of the cyclin-dependent kinase family, playing a crucial role in regulating cell cycle progression by partnering with D-type cyclins to control cell growth during the G1 phase. The function of CDK4 is essential for the phosphorylation of key substrates that drive the cell cycle forward, making CDK4 a significant focus in cancer research and studies of cellular proliferation. The activity of CDK4 is modulated through interaction with cyclins, which enhance kinase activity, and inhibitors like p16, which suppress function. Additionally, the activation of CDK4-cyclin complexes involves phosphorylation by Cdk-activating proteins (CAK), highlighting the sophisticated regulatory mechanisms governing cell cycle progression. |
| Product Image Gallery | |
 Western blot analysis of extracts on NIH/3T3, COS7 cells, using CDK4 Mouse Monoclonal Antibody (EAB21265) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
 Immunohistochemical analysis of paraffin-embedded Human kidney cancer tissue, using CDK4 Mouse Monoclonal Antibody (EAB21265) at 1:200 dilution.
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