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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IHC-P, IF/ICC, FC | 78 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IHC-P=1:50-200 IF/ICC=1:50-200 FC=1:10-100 |
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| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | 5-Lipoxygenase/5-LO Rabbit Monoclonal Antibody detects endogenous levels of 5-Lipoxygenase/5-LO protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
5-lipoxygenase/5-LO plays a crucial role in the biosynthesis of leukotrienes, which are lipid mediators involved in inflammatory responses, particularly in conditions such as asthma and allergic reactions. 5-LO catalyzes the initial steps in the conversion of arachidonic acid into leukotrienes, which induce bronchoconstriction and increase vascular permeability, contributing to the pathophysiology of various inflammatory diseases. 5-LO localization within cells varies depending on cell type and activation state. In activated polymorphonuclear leukocytes, 5-LO translocates from the cytosol to the nuclear envelope in a calcium-dependent manner, while in alveolar macrophages, 5-LO predominantly resides in the nucleus. Upon activation, intranuclear 5-LO associates with the nuclear membrane, a process influenced by interactions with specific signaling molecules, phosphorylation events, and a unique nuclear localization signal encoded at the amino terminus. |
| Product Image Gallery | |
 Western blot analysis of extracts on K562, Hela, Mouse liver, Mouse lung cells, using 5-Lipoxygenase/5-LO Rabbit Monoclonal Antibody (EAB22214) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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