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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, IF/ICC, FC, ELISA | 90 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:1000-5000 IHC-P=1:100-500 IF/ICC=1:100-500 FC=1:100-500 |
|
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | A20/TNFAIP3 Mouse Monoclonal Antibody detects endogenous levels of A20/TNFAIP3 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
A20 also known as TNF alpha induced protein 3, TNFAIP3 is a critical Cys2/Cys2 zinc finger protein that plays a vital role in regulating inflammatory responses. A20 is primarily located in the cytoplasm and is induced by inflammatory stimuli such as tumor necrosis factor (TNF) and interleukin 1 (IL-1). A20 functions as a negative regulator of nuclear factor κ B (NFκB) signaling, which is essential for controlling immune responses and preventing excessive inflammation. By inhibiting NFκB activation, A20 helps terminate inflammatory signals, maintaining cellular homeostasis and preventing potential tissue damage. A20 structure, particularly seven zinc finger domains, is crucial for function, as only four domains are necessary for in vitro inhibition of TNF-induced NFκB activation. A20 interacts with several key proteins, including TRAF2, TRAF6, and IkB kinase (IKK) γ, further underscoring importance in cellular signaling pathways. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, Jurkat, HepG2 cells, using A20/TNFAIP3 Mouse Monoclonal Antibody (EAB21709) at 1:2000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
Predicted band size: 90 kDa; Observed band size: 80 kDa |
