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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG2b | Monoclonal | WB, IP, IF/ICC, ChIP, ELISA | 185 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:2000-10000 IP=1:200-1000 IF/ICC=1:200-1000 ChIP=1:200-1000 |
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| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Brg-1 Mouse Monoclonal Antibody detects endogenous levels of Brg-1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Brg-1 also known as SMARCA4 is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Brg-1 plays a crucial role in the SWI-SNF chromatin remodeling complex, which regulates gene expression through nucleosome structure remodeling in an ATP-dependent manner. This remodeling drives various cellular processes, including differentiation, proliferation, and response to external signals. Brg-1 interacts with other key SWI-SNF complex components, such as Brm (SNF2α), Ini1 (integrase interactor 1 or SNF5), BAF155, and BAF170, forming a functional core that enhances remodeling activity. These interacting proteins stabilize the complex and modulate activity, influencing the cell′s transcriptional landscape. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, 4T1, K562, HepG2, Jurkat cells, using Brg-1 Mouse Monoclonal Antibody (EAB22351) at 1:5000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
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