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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 80 | Human, Mouse |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 | |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | FBXW7/Cdc4 Rabbit Polyclonal Antibody detects endogenous levels of FBXW7/Cdc4 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Cdc4 also known as AGO, FBW7, FBXW7, FBX30, SEL10 is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. Cdc4 plays a crucial role in cell cycle regulation by targeting cyclin E for ubiquitin-mediated degradation, preventing uncontrolled cell proliferation. Cdc4 recognizes a protein that functions in the nucleus, participating in SCF (SKP1-cullin-F-box) ubiquitin ligase complex formation, essential for phosphorylation-dependent ubiquitination. Mutations in Cdc4 have been implicated in ovarian and breast cancer cell lines. Seven tandem WD40 repeats in Cdc4 enhance substrate interaction capabilities, highlighting Cdc4′s importance in maintaining cellular homeostasis and preventing cancer progression. |
| Product Image Gallery | |
 Western blot analysis of extracts on Raji cells, using FBXW7/Cdc4 Rabbit Polyclonal Antibody (EAB11608) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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