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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP | 40 | Human |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IP=1:10-100 |
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| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | TRIB3 Rabbit Monoclonal Antibody detects endogenous levels of TRIB3 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
TRIB3 also known as TRB-3, NIPK is a putative protein kinase that is induced by the transcription factor NF-kappaB. TRIB3 plays a critical role in disrupting insulin signaling by directly binding to Akt kinases, thereby inhibiting their activation. This function is particularly important as TRIB3 contributes to insulin resistance, a key factor in the development of type II diabetes. TRIB3 is highly expressed in the liver, pancreas, peripheral blood leukocytes, and bone marrow, with increased levels observed in the livers of diabetic mice compared to their wildtype counterparts. TRIB3 interacts with ATF4, inhibiting its transcriptional activation activity and regulating the activation of mitogen-activated protein kinases (MAPKs). The regulation of these pathways underscores TRIB3′s significance in metabolic processes and potential as a therapeutic target for diabetes-related conditions. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, THP-1, A431 cells, using TRIB3 Rabbit Monoclonal Antibody (EAB22798) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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