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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP, IHC-P, IF/ICC | 55 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:1000-5000 IP=1:10-100 IHC-P=1:100-500 IF/ICC=1:100-500 |
|
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | MDM2 Rabbit Monoclonal Antibody detects endogenous levels of MDM2 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
MDM2 also known as murine double minute 2 is an E3 ubiquitin-protein ligase. MDM2 plays a critical role in regulating the tumor suppressor protein p53 by targeting p53 for ubiquitination and proteasomal degradation. Nuclear localization of MDM2 is essential for interaction with p53, as MDM2 binds directly to p53′s transactivation domain, inhibiting transcriptional activity and promoting degradation. This regulation is crucial because p53 controls cell cycle progression and induces cell cycle arrest or apoptosis in response to DNA damage. Overexpression or amplification of MDM2 can lead to decreased p53 activity, allowing cells with damaged DNA to proliferate unchecked, which contributes to tumorigenesis. MDM2 is frequently overexpressed in various human cancers, making MDM2 an important focus in cancer research and a potential target for therapeutic intervention. |
| Product Image Gallery | |
 Western blot analysis of extracts on A431, Raji, C2C12 cells, using MDM2 Rabbit Monoclonal Antibody (EAB21150) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 55 kDa; Observed band size: 90 kDa |
