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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IHC-P, IF/ICC | 70 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:1000-5000 IHC-P=1:100-500 IF/ICC=1:100-500 |
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| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Keap1 Rabbit Monoclonal Antibody detects endogenous levels of Keap1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Keap1 (kelch-like ECH-associated protein 1) also known as INrf2, KLHL19 is a stress sensing adaptor for the Cullin3 (Cul3)-dependent E3 ubiquitin ligase complex that negatively regulates Nrf2 (NF-E2-related factor 2). This regulation is vital because Nrf2 is a key transcription factor that mediates the expression of cytoprotective genes in response to oxidative stress and electrophilic insults. Under normal conditions, Keap1 maintains low levels of Nrf2 by promoting ubiquitination and subsequent degradation, thereby preventing excessive activation of protective genes. During oxidative stress, modifications to specific cysteine residues in Keap1, particularly Cys 273 and Cys 288, lead to a conformational change that releases Nrf2, allowing nuclear translocation and target gene activation. |
| Product Image Gallery | |
 Western blot analysis of extracts on HEK293, Raw264.7 cells, using Keap1 Rabbit Monoclonal Antibody (EAB22840) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
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