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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG2b | Monoclonal | WB, IF/ICC, ELISA | 45 | Human, Mouse, Rat, Pig |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IF/ICC=1:100-500 |
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| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Caspase-1 Mouse Monoclonal Antibody detects endogenous levels of Caspase-1 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Caspase-1 also known as CASP-1 is a member of the cysteine-aspartic acid protease (caspase) family. Caspase-1 plays a crucial role in the maturation of pro-inflammatory cytokines interleukin-1β (IL-1β) and interleukin-18 (IL-18) through the proteolytic cleavage of their inactive precursors. Post-translational modifications of caspase-1, particularly autocatalytic cleavage into the p20 and p10 subunits, are essential for activation and subsequent enzymatic activity, which drives inflammatory signaling and programmed cell death. The structural integrity and regulation of caspase-1 through these modifications are vital for maintaining cellular homeostasis and responding to pathological stimuli. Dysregulation of caspase-1 activity has been implicated in various diseases, including chronic inflammation, septic shock, impaired wound healing, and certain leukemias, highlighting significance in both normal physiology and disease mechanisms. |
| Product Image Gallery | |
 Western blot analysis of extracts on THP-1 cells, using Caspase-1 Mouse Monoclonal Antibody (EAB21521) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
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