www.ebiocell.com
| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Polyclonal | WB, ELISA | 207 | Human |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 | |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | PHIP Rabbit Polyclonal Antibody detects endogenous levels of PHIP protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
PHIP also known as ndrp, WDR11 is a crucial 1,821 amino acid protein characterized by eight N-terminal WD40 repeats and two bromodomains. PHIP is predominantly expressed in skeletal muscle, where PHIP localizes to both cytosol and nucleus, as well as in primary β cells, where PHIP is found in the nucleus. PHIP functions as a transcriptional activator and plays a significant role in insulin signaling by interacting with members of the insulin receptor substrate (IRS) family, particularly IRS-1 and IRS-2. These interactions are vital for mediating insulin receptor signaling, which is essential for proliferation and survival of insulin-producing β cells. PHIP′s association with the PH domain of IRS-1 suggests PHIP may serve as a critical link between IRS-1 and insulin receptors, thereby influencing regulation of insulin signaling pathways. |
| Product Image Gallery | |
 Western blot analysis of extracts on Jurkat cells, using PHIP Rabbit Polyclonal Antibody (EAB12112) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
|
