www.ebiocell.com
| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, ELISA | 47 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:1000-5000 IHC-P=1:100-500 |
|
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Smad7 Mouse Monoclonal Antibody detects endogenous levels of Smad7 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Smad7 is a nuclear protein that binds the E3 ubiquitin ligase SMURF2. Smad7 plays a crucial role in the regulation of TGFβ (transforming growth factor beta) signaling pathways, acting as a negative regulator that inhibits the phosphorylation of receptor-regulated Smads, thereby preventing their activation and subsequent transcriptional activity. This function is vital for maintaining cellular homeostasis and preventing excessive signaling that can lead to pathological conditions such as fibrosis and cancer. The ability of Smad7 to modulate TGFβ signaling highlights its importance in various biological processes, including development, immune response, and tissue repair. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, Mouse heart, Rat heart cells, using Smad7 Mouse Monoclonal Antibody (EAB11897) at 1:2000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
 Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue, using Smad7 Mouse Monoclonal Antibody (EAB11897) at 1:200 dilution.
|
