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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG2b | Monoclonal | WB, IHC-P, IF/ICC, ELISA | 48, 54 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IHC-P=1:100-500 IF/ICC=1:100-500 |
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| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Cytokeratin 8/18 Mouse Monoclonal Antibody detects endogenous levels of Cytokeratin 8/18 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Cytokeratin 8(CK8) is an intermediated filament protein with a molecular weight of 52 KD. It is a member of cytokeratin type II family. Cytokeratin 18(CK18) is an intermediated filament protein with a molecular weight of 45 KD, belongs to type I cytokeratin family. They are widely expressed in monolayer epithelial cells of thyroid , breast , gastrointestinal tract and respiratory tract. Cytokeratin 8/18 is often used as a useful marker to identify adenocarcinoma from simple epithelial cells.Cytokeratin 8/18 pair are normally expressed in simple epithelia, but not in stratified epithelial cells. Research indicates that squamous cell carcinomas derived from stratified epithelia show abnormal expression of Cytokeratin 8/18, although it is not known whether these proteins contribute to the malignant phenotype of the cells. Expression of Cytokeratin 8/18 in oral squamous cell carcinomas is an independent prognostic marker that indicates a poor prognosis. |
| Product Image Gallery | |
 Western blot analysis of extracts on HCT116, SW480, HepG2 cells, using Cytokeratin 8/18 Mouse Monoclonal Antibody (EAB12167) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
 Immunohistochemical analysis of paraffin-embedded Human liver tissue, using Cytokeratin 8/18 Mouse Monoclonal Antibody (EAB12167) at 1:200 dilution.
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