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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, IF/ICC, FC, ELISA | 36 | Human, Mouse, Rat, Monkey, Rabbit |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IHC-P=1:100-500 IF/ICC=1:100-500 FC=1:50-200 |
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| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | RANKL/TRANCE/TNFSF11 Mouse Monoclonal Antibody detects endogenous levels of RANKL/TRANCE/TNFSF11 protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
RANKL also known as TRANCE, CD254, TNFSF11 is a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. RANKL plays a pivotal role in bone remodeling and immune system regulation. By binding to its receptor RANK on osteoclasts and dendritic cells, RANKL activates critical signaling pathways such as NF-κB and JNK, leading to osteoclast differentiation and activation. This interaction is essential for normal bone resorption and remodeling processes; however, dysregulation of RANKL expression is associated with pathological conditions like osteoporosis, rheumatoid arthritis, and bone metastasis. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, HL60, Jurkat, SW480 cells, using RANKL/TRANCE/TNFSF11 Mouse Monoclonal Antibody (EAB22347) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
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