| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Mouse IgG1 | Monoclonal | WB, IHC-P, IF/ICC, FC, ELISA | 22 | Human |
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB(Westem Blotting) IHC-P(Immunohistochemistry-Paraffin) IF/ICC(Immunofluorescence/Immunocytochemistry) ELISA(Enzyme-linked Immunosorbent Assay) |
WB=1:500-2000 IHC-P=1:100-500 IF/ICC=1:100-500 FC=1:50-200 |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | BID Mouse Monoclonal Antibody detects endogenous levels of BID protein. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
BID is a member of the BCL-2 family of cell death regulators. BID plays a significant role in the regulation of apoptosis, acting as a pro-apoptotic factor that can promote cell death in response to various cellular signals. BID′s importance lies in its ability to dimerize with anti-apoptotic proteins such as Bcl-2 and Bcl-x, thereby counteracting their protective effects and facilitating the apoptotic process. The presence of a BH3 domain in BID is essential for function, allowing interactions with these proteins and influencing the balance between cell survival and death. BID is predominantly localized in the cytosol but can also be found in membrane fractions, highlighting a dynamic role in cellular signaling pathways. |
| Product Image Gallery | |
 Western blot analysis of extracts on Hela, HepG2, Jurkat, HEK293, A431 cells, using BID Mouse Monoclonal Antibody (EAB21225) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.
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