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| Host / Isotype | Clonality | Applications | MW (kDa) | Reactivity |
| Rabbit IgG | Monoclonal | WB, IP, IHC-P, IF/ICC | 32 | Human, Mouse, Rat |
| Applications Dilutions | |
| The application notes include recommended starting dilutions;optimal dilutions/concentrations should be detemined by the end user. | |
| WB=1:500-2000 IP=1:10-100 IHC-P=1:100-500 IF/ICC=1:100-500 |
|
| Application Key | WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin |
| Reactivity Key | H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish |
| Product Information | |
| Conjugate | Unconjugate |
| Specificity | Cleaved Caspase-3 (Asp175) Rabbit Monoclonal Antibody detects endogenous levels of fragment of activated Caspase-3 protein resulting from cleavage adjacent to Asp175. |
| Purification | Affinity purification |
| Concentration | 1mg/ml |
| Format | Liquid |
| Formulation | In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol. |
| Shipping | Gel Pack |
| Storage | Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks. |
| UniProt ID | |
| Entrez Gene ID | |
| Product Description | |
Caspase-3 also known as SCA-1, Yama, CASP3 is a crucial aspartate-specific cysteine protease within the ICE subfamily of caspases. Caspase-3 plays a pivotal role in the execution phase of apoptosis, the programmed cell death process essential for maintaining cellular homeostasis and eliminating damaged or unwanted cells. Upon activation, caspase-3 cleaves key substrates like PARP, leading to the dismantling of cellular components necessary for apoptosis. Additionally, caspase-3 is involved in activating other caspases, including caspase-6, -7, and -9, thereby amplifying the apoptotic signal. Caspase-3′s structural domains facilitate localization and interaction within the cytoplasm, making caspase-3 a key player in various physiological and pathological contexts. |
| Product Image Gallery | |
 Western blot analysis of extracts on NIH/3T3 cells, using Cleaved Caspase-3 (Asp175) Rabbit Monoclonal Antibody (EAB21377) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Predicted band size: 32 kDa; Observed band size: 35,17,19 kDa |
