Monoclonal Antibodies - DYKDDDDK-Tag Mouse Monoclonal Antibody

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DYKDDDDK-Tag Mouse Monoclonal Antibody

Catalog #：EAB10043

Datasheet

	SKU-Pack Size	Availability	Size	Price
	EAB10043-100UL	In Stock	100ul	€115.70

If you want to get a larger packsize, Please contact your local distributor or
email us at info@ebiocell.com

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	Betaclonal	Phone：+34 (0)91 471 6763
	29/1 Lomonosovsky Pr,	E-mail：tech@betaclonal.com
Spain	Madrid, Spain	Web：www.betaclonal.com

Product Information
Applications	WB, IP, IF/ICC
Species Reactivity	Species independent
Host / Isotype	Mouse IgG1
Clonality	Monoclonal
Applications Dilutions	WB=1:10000-50000　 IP=1:100-500　 IF/ICC=1:500-3000
MW (kDa)	N/A
Conjugate	Unconjugate
Specificity	DYKDDDDK-Tag Mouse Monoclonal Antibody detects endogenous levels of DYKDDDDK-Tag fusion protein.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	Flag-Tag; DDDDK-Tag; DYKDDDDK-Tag
Gene Aliases	FLAG
UniProt ID	N/A
Entrez Gene ID	N/A

Product Description

DYKDDDDK-Tag (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence.

Product Image Gallery
Western blot of DYKDDDDK-Tag fusion protein on 293T cells, Using Mouse Monoclonal Antibody (EAB10043) at 1:10000, 1:20000 and 1:40000 dilution. Immunofluorescence analysis of 293 cell, using HA-Tag Mouse Monoclonal Antibody (EAB10043) (red) at 1:2000 dilution.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol