Polyclonal Antibodies - Phospho-Smad1 (Ser465) Rabbit Polyclonal Antibody

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Phospho-Smad1 (Ser465) Rabbit Polyclonal Antibody

Catalog #：EAB10064

Datasheet

SKU-Pack Size	Availability	Size	Price
EAB10064-30UL	In Stock	30ul	€141.70
EAB10064-100UL	In Stock	100ul	€297.70
EAB10064-200UL	In Stock	200ul	€479.70

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email us at info@ebiocell.com

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	OlaSciences	Phone：+33 (0) 9 77 4001 556
	87, rue des Suisses 65000 Nanterre,	E-mail：ola@olasciences.com
France	France	Web：www.olasciences.com

Product Information
Applications	WB, IHC-P, IF, ELISA
Species Reactivity	Human, Mouse, Rat
Host / Isotype	Rabbit IgG
Clonality	Polyclonal
Applications Dilutions	WB=1:500-2000　 IHC-P=1:50-300　 IF=1:50-300
MW (kDa)	52
Conjugate	Unconjugate
Specificity	Phospho-Smad1 (Ser465) Rabbit Polyclonal Antibody detects endogenous levels of Smad1 only when phosphorylated at Ser465.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	SMAD family member 1; BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1; SMAD1
Gene Aliases	SMAD1
UniProt ID	Q15797
Entrez Gene ID	4086

Product Description

Smad1 also known as MADH1, JV4-1, MADR1 is an intracellular signal transducer and transcriptional modulator involved in the Bone Morphogenetic Protein (BMP) pathway, part of the Transforming Growth Factor-beta (TGF-β) superfamily. Upon activation by BMP receptors, Smad1 is phosphorylated and forms a complex with Smad4, translocating from the cytoplasm to the nucleus. This nuclear localization is crucial because Smad1 regulates the expression of target genes that control cell proliferation, differentiation, and apoptosis. Smad1 function is essential for embryonic development, tissue homeostasis, and bone formation. Dysregulation of Smad1 signaling has been implicated in various diseases, including cancer and fibrosis.

Product Image Gallery
Western blot analysis of extracts on Hela cells treated with Serum, using Phospho-Smad1 (Ser465) Rabbit Polyclonal Antibody (EAB10064) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide. Predicted band size: 52 kDa; Observed band size: 60 kDa

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol