Polyclonal Antibodies - Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) Rabbit Polyclonal Antibody

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Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) Rabbit Polyclonal Antibody

Catalog #：EAB14559

Datasheet

	SKU-Pack Size	Availability	Size	Price
	EAB14559-100UL	In Stock	100ul	$343.50

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	EbioCell	Phone：(540) 808-3925
	Imperial Business Park 4819 Emperor Boulevard,	E-mail：order@ebiocell.com
United States	Suite 408 Durham, NC 27703, USA	Web：www.ebiocell.com

Product Information
Applications	WB, IHC-P, IF/ICC
Species Reactivity	Human, Mouse
Host / Isotype	Rabbit IgG
Clonality	Polyclonal
Applications Dilutions	WB=1:500-2000 IHC-P=1:50-300　 IF/ICC=1:50-300
MW (kDa)	48, 52
Conjugate	Unconjugate
Specificity	Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) Rabbit Polyclonal Antibody detects endogenous levels of Smad2 protein when phosphorylated at Ser465 and Ser467. also recognizes endogenous levels of Smad3 protein when phosphorylated at Ser423 and Ser425.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	SMAD family member 2; SMAD family member 3; JV18; LDS6; CHTD8; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2; LDS3; mad3; LDS1C; MADH3; JV15-2; hMAD-3; hSMAD3; HSPC193; HsT17436; SMAD2; SMAD3
Gene Aliases	SMAD2/SMAD3
UniProt ID	P84022, Q15796
Entrez Gene ID	4088, 4087

Product Description

Smad2 and Smad3 also known as MAD2 and MAD3 respectively, are essential components of the Transforming Growth Factor-beta (TGFβ) signaling pathway. These proteins interact directly with Smad4 (DPC4), forming complexes that translocate to the nucleus to regulate gene transcription involved in cell proliferation, differentiation, and apoptosis. Additionally, Smad2/3 interact with various transcription factors and co-regulators, such as FOXH1 and p300, to modulate the expression of target genes. The interaction between Smad2/3 and TGFβ receptors is crucial for the phosphorylation and activation of these Smad proteins, facilitating their role in cellular homeostasis and development.

Product Image Gallery
Western blot analysis of extracts on Hela cells treated with PMA, using Phospho-Smad2 (Ser465/467)/Smad3 (Ser423/425) Rabbit Polyclonal Antibody (EAB14559) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide. Predicted band size: 48,52 kDa; Observed band size: 52,60 kDa

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol