Polyclonal Antibodies - Phospho-Aurora B (Thr232) Rabbit Polyclonal Antibody

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Phospho-Aurora B (Thr232) Rabbit Polyclonal Antibody

Catalog #：EAB10639

Datasheet

SKU-Pack Size	Availability	Size	Price
EAB10639-30UL	In Stock	30ul	￡119.90
EAB10639-100UL	In Stock	100ul	￡251.90
EAB10639-200UL	In Stock	200ul	￡405.90

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email us at info@ebiocell.com

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	CUlabSciences	Phone：+44 (0) 1232 330008
	Cambridge House St Thomas' Place Ely,	E-mail：sales@culabsciences.co.uk
United Kingdom	Cambridge CB27 9RD UK	Web：www.culabsciences.co.uk

Product Information
Applications	WB, IHC-P, IF/ICC, ELISA
Species Reactivity	Human, Mouse, Rat, Monkey
Host / Isotype	Rabbit IgG
Clonality	Polyclonal
Applications Dilutions	WB=1:500-2000　 IHC-P=1:50-300　 IF/ICC=1:50-300
MW (kDa)	39
Conjugate	Unconjugate
Specificity	Phospho-Aurora B (Thr232) Rabbit Polyclonal Antibody detects endogenous levels of Aurora B only when phosphorylated at Thr232.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	Aurora kinase B; AIK2; AIM1; ARK2; AurB; IPL1; STK5; AIM-1; ARK-2; STK-1; STK12; PPP1R48; aurkb-sv1; aurkb-sv2; AURKB
Gene Aliases	AURKB
UniProt ID	Q96GD4
Entrez Gene ID	9212

Product Description

Aurora B also known as ARK-2, AURKB is a member of the aurora kinase subfamily of serine/threonine kinases. Aurora B plays a crucial role in regulating centrosome separation, bipolar spindle assembly, and chromosome segregation during mitosis, making Aurora B essential for proper cell division and genomic stability. Aurora B is predominantly localized in the nucleus, where Aurora B associates with distinct components of the mitotic machinery, such as the spindle poles and midbody, highlighting Aurora B′s importance in the orchestration of mitotic events. Notably, Aurora B protein levels peak during the S and G2/M phases of the cell cycle, indicating Aurora B′s active involvement in cell cycle progression, while Aurora B′s unique genetic locus suggests distinct functions compared to Aurora A.

Product Image Gallery
Western blot analysis of extracts on COS7 cells treated with Nocodazole, using Phospho-Aurora B (Thr232) Rabbit Polyclonal Antibody (EAB10639) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol