Polyclonal Antibodies - Phospho-Cofilin (Ser3) Rabbit Polyclonal Antibody

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Phospho-Cofilin (Ser3) Rabbit Polyclonal Antibody

Catalog #：EAB10696

Datasheet

SKU-Pack Size	Availability	Size	Price
EAB10696-30UL	In Stock	30ul	€141.70
EAB10696-100UL	In Stock	100ul	€297.70
EAB10696-200UL	In Stock	200ul	€479.70

If you want to get a larger packsize, Please contact your local distributor or
email us at info@ebiocell.com

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	Betaclonal	Phone：+34 (0)91 471 6763
	29/1 Lomonosovsky Pr,	E-mail：tech@betaclonal.com
Spain	Madrid, Spain	Web：www.betaclonal.com

Product Information
Applications	WB, IHC-P, IF, ELISA
Species Reactivity	Human, Mouse, Rat
Host / Isotype	Rabbit IgG
Clonality	Polyclonal
Applications Dilutions	WB=1:500-2000　 IHC-P=1:50-300　 IF=1:50-300
MW (kDa)	19
Conjugate	Unconjugate
Specificity	Phospho-Cofilin (Ser3) Rabbit Polyclonal Antibody detects endogenous levels of cofilin only when phosphorylated at Ser3.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	CFL; CFL1; Cof; cofilin; HEL-S-15; n-cofilin; Cofilin1; 18 kDa phosphoprotein; p18; CFL-1; Cofilin-1
Gene Aliases	CFL1
UniProt ID	P23528
Entrez Gene ID	1072

Product Description

The protein encoded by this gene can polymerize and depolymerize F-actin and G-actin in a pH-dependent manner. Increased phosphorylation of this protein by LIM kinase aids in Rho-induced reorganization of the actin cytoskeleton. Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus.

Product Image Gallery

Western blot analysis of extracts on NIH/3T3 cells treated with Paclitaxel, using Phospho-Cofilin (Ser3) Rabbit Polyclonal Antibody (EAB10696) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide.

Western blot analysis of extracts on different cells, using Phospho-Cofilin (Ser3) Rabbit Polyclonal Antibody (EAB10696) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Lane1: U87MG
Lane2: U251

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol