Polyclonal Antibodies - Phospho-eIF4E (Ser209) Rabbit Polyclonal Antibody

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Phospho-eIF4E (Ser209) Rabbit Polyclonal Antibody

Catalog #：EAB10729

Datasheet

SKU-Pack Size	Availability	Size	Price
EAB10729-30UL	In Stock	30ul	€141.70
EAB10729-100UL	In Stock	100ul	€297.70
EAB10729-200UL	In Stock	200ul	€479.70

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email us at info@ebiocell.com

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	SBT BioChem Srl	Phone：+39 05 7156 8147
	Via Ponte di Bagnolo, 811 Bologna,	E-mail：info@sbtbiochem.it
Italy	Italy	Web：www.sbtbiochem.it

Product Information
Applications	WB, IHC-P, IF, ELISA
Species Reactivity	Human, Mouse, Rat
Host / Isotype	Rabbit IgG
Clonality	Polyclonal
Applications Dilutions	WB=1:500-2000　 IHC-P=1:50-300　 IF=1:50-300
MW (kDa)	25
Conjugate	Unconjugate
Specificity	Phospho-eIF4E (Ser209) Rabbit Polyclonal Antibody detects endogenous levels of eIF4E only when phosphorylated at Ser209.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	Eukaryotic translation initiation factor 4E; CBP; EIF4F; AUTS19; EIF4E1; eIF-4E; EIF4EL1; EIF4E
Gene Aliases	EIF4E
UniProt ID	P06730
Entrez Gene ID	1977

Product Description

eIF4E is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants.

Product Image Gallery
Western blot analysis of extracts on 293 cells treated with Anisomycin, using Phospho-eIF4E (Ser209) Rabbit Polyclonal Antibody (EAB10729) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide. Immunohistochemistry analysis of paraffin-embedded Human breast carcinoma tissue, using Phospho-eIF4E (Ser209) Rabbit Polyclonal Antibody (EAB10729) at 1:100 dilution.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol