Polyclonal Antibodies - Phospho-Smad3 (Ser423/425) Rabbit Polyclonal Antibody

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Phospho-Smad3 (Ser423/425) Rabbit Polyclonal Antibody

Catalog #：EAB10848

Datasheet

SKU-Pack Size	Availability	Size	Price
EAB10848-30UL	In Stock	30ul	€141.70
EAB10848-100UL	In Stock	100ul	€297.70
EAB10848-200UL	In Stock	200ul	€479.70

If you want to get a larger packsize, Please contact your local distributor or
email us at info@ebiocell.com

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	Cambiotech	Phone：+44 (0) 188 448801
	Lanwades Business Park,	E-mail：info@cambiotech.com
Ireland	Kobenhavn K, Ireland	Web：www.cambiotech.com

Product Information
Applications	WB, IHC-P, ELISA
Species Reactivity	Human, Mouse, Rat
Host / Isotype	Rabbit IgG
Clonality	Polyclonal
Applications Dilutions	WB=1:500-2000　 IHC-P=1:50-300
MW (kDa)	48
Conjugate	Unconjugate
Specificity	Phospho-Smad3 (Ser423/425) Rabbit Polyclonal Antibody detects endogenous levels of Smad3 when phosphorylated at Ser423/425.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	SMAD family member 3; LDS3; mad3; LDS1C; MADH3; JV15-2; hMAD-3; hSMAD3; HSPC193; HsT17436; SMAD3
Gene Aliases	SMAD3
UniProt ID	P84022
Entrez Gene ID	4088

Product Description

Smad3 also known as LDS3 and MADH3 is a critical intracellular mediator in the Transforming Growth Factor-beta (TGF-β) signaling pathway. Smad3 playing a pivotal role in regulating gene expression related to cell proliferation, differentiation, apoptosis, and extracellular matrix production. By transducing signals from the cell membrane to the nucleus, Smad3 facilitates the transcription of target genes that are essential for maintaining cellular homeostasis and tissue development. Dysregulation of Smad3 activity is implicated in various pathological conditions, including fibrosis, cancer progression, and immune response modulation, making Smad3 a significant target for research and therapeutic intervention.

Product Image Gallery
Western blot analysis of extracts on Hela cells, using Phospho-Smad3 (Ser423/425) Rabbit Polyclonal Antibody (EAB10848) at 1:1000 dilution. The lane on the left is blocked with the phospho peptide. Predicted band size: 48 kDa; Observed band size: 50 kDa

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol