Recombinant Antibodies - Phospho-Smad5 (Ser463/465) Rabbit Monoclonal Antibody

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Phospho-Smad5 (Ser463/465) Rabbit Monoclonal Antibody

Catalog #：EAB22601

Datasheet

	SKU-Pack Size	Availability	Size	Price
	EAB22601-100UL	In Stock	100ul	€297.70

If you want to get a larger packsize, Please contact your local distributor or
email us at info@ebiocell.com

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	OlaSciences	Phone：+33 (0) 9 77 4001 556
	87, rue des Suisses 65000 Nanterre,	E-mail：ola@olasciences.com
France	France	Web：www.olasciences.com

Product Information
Applications	WB, IHC-P, IF/ICC
Species Reactivity	Human, Mouse, Rat
Host / Isotype	Rabbit IgG
Clonality	Monoclonal
Applications Dilutions	WB=1:500-2000　 IHC-P=1:50-200　 IF/ICC=1:50-200
MW (kDa)	52
Conjugate	Unconjugate
Specificity	Phospho-Smad5 (Ser463/465) Rabbit Monoclonal Antibody detects endogenous levels of Smad1 and Smad5 only when dually phosphorylated at Ser463 and Ser465.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	SMAD family member 5; DWFC; JV5-1; MADH5; SMAD5
Gene Aliases	SMAD5
UniProt ID	Q99717
Entrez Gene ID	4090

Product Description

Smad5 is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. Smad5 plays a crucial role in the bone morphogenetic protein (BMP) signaling pathway, which is essential for various biological processes, including embryonic development, tissue homeostasis, and regulation of cell growth and differentiation. Smad5 acts as a transcription factor that transduces signals from BMPs, leading to activation of target genes involved in osteogenesis and chondrogenesis. Proper functioning of Smad5 is vital, as dysregulation of BMP signaling has been implicated in various diseases, including cancer and skeletal disorders.

Product Image Gallery
Western blot analysis of extracts on Hela, MCF7 cells, using Phospho-Smad5 (Ser463/465) Rabbit Monoclonal Antibody (EAB22601) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol