Monoclonal Antibodies - ASC Mouse Monoclonal Antibody

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ASC Mouse Monoclonal Antibody

Catalog #：EAB21727

Datasheet

	SKU-Pack Size	Availability	Size	Price
	EAB21727-100UL	In Stock	100ul	￡284.90

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	CUlabSciences	Phone：+44 (0) 1232 330008
	Cambridge House St Thomas' Place Ely,	E-mail：sales@culabsciences.co.uk
United Kingdom	Cambridge CB27 9RD UK	Web：www.culabsciences.co.uk

Product Information
Applications	WB, IHC-P, IF/ICC
Species Reactivity	Human, Rat
Host / Isotype	Mouse IgG1
Clonality	Monoclonal
Applications Dilutions	WB=1:2000-10000　 IHC-P=1:100-500 IF/ICC=1:100-500
MW (kDa)	22
Conjugate	Unconjugate
Specificity	ASC Mouse Monoclonal Antibody detects endogenous levels of ASC protein.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	PYD and CARD domain containing; ASC; TMS; TMS1; CARD5; TMS-1; PYCARD
Gene Aliases	PYCARD
UniProt ID	Q9ULZ3
Entrez Gene ID	29108

Product Description

ASC also known as TMS1, PYCARD is a critical member of the CARD-containing adaptor protein family. ASC playing a pivotal role in apoptotic signaling by mediating interactions between adaptor proteins like Apaf-1 and pro-caspases such as CASP9. Structurally, ASC comprises a Pyrin-like domain (PYD) at the N-terminus and an 87-residue caspase recruitment domain (CARD) at the C-terminus, which are essential for its function in apoptosis regulation. Upon induction of apoptosis, ASC undergoes a transformation from diffuse cytoplasmic distribution to forming punctate or spherical perinuclear aggregates, a process that can be visualized through fluorescence microscopy. This aggregation is significant in the cellular response to apoptotic stimuli, including treatments with retinoic acid and various anti-tumor drugs.

Product Image Gallery
Western blot analysis of extracts on THP-1, U937 cells, using ASC Mouse Monoclonal Antibody (EAB21727) at 1:5000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution. Predicted band size: 22 kDa; Observed band size: 20-25 kDa

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol