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Location：Home > Antibodies > Primary Antibodies > Polyclonal Antibodies
FIH Rabbit Polyclonal Antibody
Catalog #：EAB11941
Datasheet
  SKU-Pack Size Availability Size Price
EAB11941-100UL In Stock 100ul ￥1990.00
EAB11941-200UL In Stock 200ul ￥3390.00
If you want to get a larger packsize, Please contact your local distributor or
email us at info@ebiocell.com
Please select the country you are in to find your local distributor.
北京美瑞克生物科技有限公司 Phone：  +86 010-62890160 / 13691184142(微信同号)
2 / F, 128 malianwa North Road, Haidian District, E-mail：   mrkbio@163.com 
China Beijing China Website： www.mrkbio.com
Product Information
Applications WB
Species Reactivity Human, Mouse, Rat
Host / Isotype Rabbit IgG
Clonality Polyclonal
Applications Dilutions WB=1:500-2000
MW (kDa) 40
Conjugate Unconjugate
Specificity FIH Rabbit Polyclonal Antibody detects endogenous levels of FIH protein.
Purification Affinity purification
Concentration 1mg/ml
Format Liquid
Formulation In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping Gel Pack
Storage Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key

WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key

H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected
Product Bioinformatics
Synonym(s) FIH-1; FIH1; Hypoxia inducible factor 1 alpha inhibitor; HIF1AN; FIH; HIF1N
Gene Aliases HIF1AN
UniProt ID

Q9NWT6
Entrez Gene ID

55662
Product Description

Factor inhibiting HIF-1 (FIH-1) exists as a homodimer and binds to HIF-1alpha. Specifically, FIH-1 operates as an asparaginyl hydroxylase. It catalyzes the hydroxylation of the beta-carbon of Asparagine residue 803 within the carboxy terminal transactivation domain of HIF-1alpha. This hydroxylation event blocks the association of HIF-1alpha with co-activators. FIH-1 also binds to von Hippel-Lindau (VHL) tumor suppressor protein, which represses transcriptional ac-tivity of HIF-1alpha. In transiently transfected human osteosarcoma cells, FIH-1 localizes to the cytoplasm.
Product Image Gallery
Western blot analysis of extracts on different cells, using FIH Rabbit Polyclonal Antibody (EAB11941) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.
Lane1: 293T
Lane2: MCF7
Specific Protocols
>> Western Blotting Protocol >> Immunoprecipitation Protocol
>> Immunohistochemistry Protocol >> Immunofluorescence Protocol
>> Immunocytochemistry Protocol >> Flow Cytometry Protocol
>> ChIP Protocol >> ELISA Protocol
>> HPLC Protocol >> PCR Protocol
For Research Use Only, Not For Diagnostic Or Therapeutic Procedures.
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