Aiolos also known as zinc finger protein Ikaros 3, ZNFN1A3 is a member of the Ikaros family of zinc-finger proteins. Aiolos is a crucial 509 amino acid nuclear protein that plays a significant role in lymphocyte differentiation regulation, vital for proper immune system function. Mutations in Aiolos have been implicated in leukemogenesis, highlighting Aiolos′s importance in maintaining cellular integrity and function. Predominantly expressed in spleen, thymus, and peripheral blood leukocytes, Aiolos contains six C2H2-type zinc fingers that facilitate DNA binding and formation of homodimers and heterodimers with other Ikaros family members, such as Ikaros and Helios. These interactions are essential for Aiolos′s function as a transcription factor, allowing response to signaling events like T-cell activation and colocalization with DNA replication machinery components in higher-order chromatin structures.