Monoclonal Antibodies - LEF1 Mouse Monoclonal Antibody

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LEF1 Mouse Monoclonal Antibody

Catalog #：EAB21975

Datasheet

SKU-Pack Size	Availability	Size	Price
EAB21975-30UL	In Stock	30ul	€115.70
EAB21975-100UL	In Stock	100ul	€258.70

If you want to get a larger packsize, Please contact your local distributor or
email us at info@ebiocell.com

Please select the country you are in to find your local distributor.

	Cambiotech	Phone：+44 (0) 188 448801
	Lanwades Business Park,	E-mail：info@cambiotech.com
Ireland	Kobenhavn K, Ireland	Web：www.cambiotech.com

Product Information
Applications	WB, IHC-P, ELISA
Species Reactivity	Human
Host / Isotype	Mouse IgG1
Clonality	Monoclonal
Applications Dilutions	WB=1:500-2000　 IHC-P=1:100-500
MW (kDa)	44
Conjugate	Unconjugate
Specificity	LEF1 Mouse Monoclonal Antibody detects endogenous levels of LEF1 protein.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	Lymphoid enhancer binding factor 1; ECTD1; LEF-1; TCF10; ECTD17; TCF7L3; TCF1ALPHA; LEF1
Gene Aliases	LEF1
UniProt ID	Q9UJU2
Entrez Gene ID	51176

Product Description

LEF1 also known as Lymphoid enhancer binding factor 1 is a member of the TCF/LEF family of transcription factors, plays a crucial role in the Wnt signaling pathway, which is essential for various developmental processes and cellular functions. LEF1 is primarily located in the nucleus, where LEF1 interacts with β-catenin to regulate the transcription of Wnt target genes. Nuclear localization of LEF1 allows for precise control of gene expression in response to Wnt signaling, influencing cell fate decisions during embryonic development and tissue homeostasis. Dysregulation of LEF1 and associated pathways can lead to various diseases, including cancer, highlighting the importance of studying LEF1 for understanding tumorigenesis and potential therapeutic interventions.

Product Image Gallery

Western blot analysis of extracts on Jurkat, HepG2, Molt-4 cells, using LEF1 Mouse Monoclonal Antibody (EAB21975) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.

Predicted band size: 44 kDa; Observed band size: 45-60 kDa

Immunohistochemical analysis of paraffin-embedded Hhuman colon cancer tissue, using LEF1 Mouse Monoclonal Antibody (EAB21975) at 1:200 dilution.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol