Monoclonal Antibodies - SP1 Mouse Monoclonal Antibody

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SP1 Mouse Monoclonal Antibody

Catalog #：EAB22269

Datasheet

	SKU-Pack Size	Availability	Size	Price
	EAB22269-100UL	In Stock	100ul	€336.70

If you want to get a larger packsize, Please contact your local distributor or
email us at info@ebiocell.com

Please select the country you are in to find your local distributor.

	Cambiotech	Phone：+44 (0) 188 448801
	Lanwades Business Park,	E-mail：info@cambiotech.com
Ireland	Kobenhavn K, Ireland	Web：www.cambiotech.com

Product Information
Applications	WB, IHC-P, IF/ICC, FC, ELISA
Species Reactivity	Human, Mouse, Rat
Host / Isotype	Mouse IgG1
Clonality	Monoclonal
Applications Dilutions	WB=1:1000-5000　 IHC-P=1:100-500　 IF/ICC=1:100-500　 FC=1:100-500
MW (kDa)	81
Conjugate	Unconjugate
Specificity	SP1 Mouse Monoclonal Antibody detects endogenous levels of SP1 protein.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	Sp1 transcription factor; Specificity protein 1; SP1
Gene Aliases	SP1
UniProt ID	P08047
Entrez Gene ID	6667

Product Description

SP1(Specificity protein 1) is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. SP1 plays a crucial role in gene expression, cell growth, differentiation, and apoptosis. Initially identified as a factor that activates transcription from the SV40 promoter by binding to multiple GC boxes in the 21-base pair repeats, SP1′s DNA-binding specificity is conferred by three Cys2His2-type zinc finger motifs. Phosphorylation of SP1 can modulate activity and DNA-binding affinity, affecting the transcription of target genes. Located predominantly in the nucleus, SP1′s localization is essential for function as a transcriptional regulator. Dysregulation of SP1 has been implicated in various diseases, including cancer, highlighting importance in maintaining normal cellular functions.

Product Image Gallery
Western blot analysis of extracts on HEK293, NIH/3T3 cells, using SP1 Mouse Monoclonal Antibody (EAB22269) at 1:2000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution. Predicted band size: 81 kDa; Observed band size: 105 kDa

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol