Polyclonal Antibodies - Phospho-Smad2 (Ser465) Rabbit Polyclonal Antibody

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Phospho-Smad2 (Ser465) Rabbit Polyclonal Antibody

Catalog #：EAB13988

Datasheet

SKU-Pack Size	Availability	Size	Price
EAB13988-30UL	In Stock	30ul	€141.70
EAB13988-100UL	In Stock	100ul	€297.70
EAB13988-200UL	In Stock	200ul	€479.70

If you want to get a larger packsize, Please contact your local distributor or
email us at info@ebiocell.com

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	Betaclonal	Phone：+34 (0)91 471 6763
	29/1 Lomonosovsky Pr,	E-mail：tech@betaclonal.com
Spain	Madrid, Spain	Web：www.betaclonal.com

Product Information
Applications	WB, IHC-P, IF, ELISA
Species Reactivity	Human, Mouse, Rat
Host / Isotype	Rabbit IgG
Clonality	Polyclonal
Applications Dilutions	WB=1:500-2000　 IHC-P=1:50-300　 IF=1:50-300
MW (kDa)	52
Conjugate	Unconjugate
Specificity	Phospho-Smad2 (Ser465) Rabbit Polyclonal Antibody detects endogenous levels of Smad2 only when phosphorylated at Ser465.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	SMAD family member 2; SMAD family member 3; JV18; LDS6; CHTD8; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2; LDS3; mad3; LDS1C; MADH3; JV15-2; hMAD-3; hSMAD3; HSPC193; HsT17436; SMAD2; SMAD3
Gene Aliases	SMAD2
UniProt ID	Q15796
Entrez Gene ID	4087

Product Description

Smad2/Smad3 also known as MADR2/MADH3 respectively, are essential components of the Transforming Growth Factor-beta (TGFβ) signaling pathway. These proteins interact directly with Smad4 (DPC4), forming complexes that translocate to the nucleus to regulate gene transcription involved in cell proliferation, differentiation, and apoptosis. Additionally, Smad2/3 interact with various transcription factors and co-regulators, such as FOXH1 and p300, to modulate the expression of target genes. The interaction between Smad2/3 and TGFβ receptors is crucial for the phosphorylation and activation of these Smad proteins, facilitating their role in cellular homeostasis and development.

Product Image Gallery
Western blot analysis of extracts on Mouse liver cells, using Phospho-Smad2 (Ser465) Rabbit Polyclonal Antibody (EAB13988) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol