PCK1 also known as PEPCK-C, PEPCK1 is a crucial enzyme in the gluconeogenesis pathway, responsible for the synthesis of glucose from non-carbohydrate sources in liver cells, thereby maintaining blood glucose levels during fasting and metabolic stress. Structurally, PCK1 interacts with several key proteins, including the transcription factor FOXO1 and the coactivator PGC-1α, which regulate its expression in response to hormonal signals like insulin and glucagon. These interactions are essential for the dynamic control of glucose production and metabolic homeostasis. Dysregulation of PCK1 and its interacting partners is closely associated with metabolic disorders such as type 2 diabetes and obesity, highlighting its significance as a therapeutic target.