Polyclonal Antibodies - PCK1 Rabbit Polyclonal Antibody

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PCK1 Rabbit Polyclonal Antibody

Catalog #：EAB14360

Datasheet

SKU-Pack Size	Availability	Size	Price
EAB14360-30UL	In Stock	30ul	€115.70
EAB14360-100UL	In Stock	100ul	€258.70
EAB14360-200UL	In Stock	200ul	€440.70

If you want to get a larger packsize, Please contact your local distributor or
email us at info@ebiocell.com

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	Dianost	Phone：+49 (0) 40 65472-053
	Bullenkoppel 11， 23896 Poggensee,	E-mail：ola@olasciences.com
Germany	Germany	Web：www.olasciences.com

Product Information
Applications	WB, IHC-P, IF, ELISA
Species Reactivity	Human, Mouse, Rat
Host / Isotype	Rabbit IgG
Clonality	Polyclonal
Applications Dilutions	WB=1:500-2000　 IHC-P=1:50-300　 IF=1:50-300
MW (kDa)	69
Conjugate	Unconjugate
Specificity	PCK1 Rabbit Polyclonal Antibody detects endogenous levels of PCK1 protein.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	PCKDC; PEPCK1; PEPCKC; PCK1; PEPCK-1; Phosphoenolpyruvate carboxykinase, cytosolic [GTP]
Gene Aliases	PCK1
UniProt ID	P35558
Entrez Gene ID	5105

Product Description

PCK1 (also designated PEPCK-C, PEPCK1) is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. Defects in this gene are a cause of cytosolic phosphoenolpyruvate carboxykinase deficiency. A mitochondrial isozyme of the encoded protein also has been characterized.

Product Image Gallery

Western blot analysis of extracts on MCF7, Hela cells, using PCK1 Rabbit Polyclonal Antibody (EAB14360) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue, using PCK1 Rabbit Polyclonal Antibody (EAB14360) at 1:100 dilution.

Immunohistochemical analysis of paraffin-embedded Rat liver tissue, using PCK1 Rabbit Polyclonal Antibody (EAB14360) at 1:100 dilution.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol