DAG1 also known as Dystroglycan is a central component of dystrophin-glycoprotein complex that links the extracellular matrix and the cytoskeleton in the skeletal muscle. DAG1 plays a crucial role as a cell surface receptor for several extracellular matrix molecules, including laminins, Agrin, and Perlecan, which are essential for basement membrane formation during early development and laminin organization on cell surfaces. α-DAG1′s location at cell membranes facilitates interaction with dystrophin and utrophin, forming dystrophin-associated protein complex and utrophin-associated protein complex, respectively. These complexes maintain structural integrity of muscle cells and cluster nicotinic acetylcholine receptors at neuromuscular junctions, essential for effective synaptic transmission.