Polyclonal Antibodies - NFS1 Rabbit Polyclonal Antibody

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NFS1 Rabbit Polyclonal Antibody

Catalog #：EAB11095

Datasheet

SKU-Pack Size	Availability	Size	Price
EAB11095-30UL	In Stock	30ul	￡97.90
EAB11095-100UL	In Stock	100ul	￡218.90
EAB11095-200UL	In Stock	200ul	￡372.90

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	CUlabSciences	Phone：+44 (0) 1232 330008
	Cambridge House St Thomas' Place Ely,	E-mail：sales@culabsciences.co.uk
United Kingdom	Cambridge CB27 9RD UK	Web：www.culabsciences.co.uk

Product Information
Applications	WB, ELISA
Species Reactivity	Human, Rat
Host / Isotype	Rabbit IgG
Clonality	Polyclonal
Applications Dilutions	WB=1:500-2000
MW (kDa)	50
Conjugate	Unconjugate
Specificity	NFS1 Rabbit Polyclonal Antibody detects endogenous levels of NFS1 protein.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	Nitrogen fixation 1; Cysteine desulfurase; IscS; NIFS; COXPD52; HUSSY-08; NFS1
Gene Aliases	NFS1
UniProt ID	Q9Y697
Entrez Gene ID	9054

Product Description

NFS1 also known as nitrogen fixation 1 is a crucial enzyme that plays a significant role in the biosynthesis of iron-sulfur (Fe-S) clusters, which are vital cofactors for numerous biological processes. NFS1 is primarily located in the cytoplasm and mitochondria, with localization being influenced by cytosolic pH levels. This dual localization allows NFS1 to participate in various metabolic pathways, including the synthesis of Fe-S clusters that are essential for electron transfer in oxidative phosphorylation and enzymatic reactions in key metabolic enzymes such as aconitase and dehydratases. NFS1′s ability to supply inorganic sulfur for Fe-S cluster assembly underscores its importance in maintaining cellular function and integrity, particularly in energy metabolism and DNA repair mechanisms.

Product Image Gallery
Western blot analysis of extracts on Hela, HepG2 cells, using NFS1 Rabbit Polyclonal Antibody (EAB11095) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol