ACSL1(Acyl-CoA synthetase long chain family member 1) is an isozyme of the long-chain fatty-acid-coenzyme A ligase family. ACSL1 plays a crucial role in lipid metabolism, specifically in the activation of long-chain fatty acids to acyl-CoA derivatives, which are essential for various metabolic pathways, including the synthesis of cellular lipids and the β-oxidation process. This activation allows fatty acids to be utilized for energy production and the formation of important biomolecules such as triacylglycerols, phospholipids, and cholesteryl esters. ACSL1 is predominantly expressed in the liver and exhibits a preference for substrates like palmitoleate, oleate, and linoleate, highlighting its significance in maintaining lipid homeostasis and energy balance within the body. ACSL1′s ability to interact with various long-chain fatty acids underscores its importance in metabolic regulation and potential implications in metabolic disorders.