Arginase-1 also known as Arg-1,  ARG1 is a crucial enzyme primarily expressed in the liver, where Arginase-1 plays a vital role in the urea cycle by catalyzing the hydrolysis of arginine into ornithine and urea, thereby facilitating the removal of excess nitrogen from the body. Structurally, Arginase-1 functions as a homotrimeric protein and contains a binuclear manganese cluster essential for enzymatic activity. Understanding Arginase 1 function is important as Arginase-1 not only regulates amino acid metabolism but also influences various physiological and pathological processes, including wound healing, immune response, and cancer progression.