BAF53 also known as ACTL6 is a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. BAF53 plays a significant role in regulating gene expression by facilitating ATP-dependent alterations in chromatin structure. BAF53 is primarily located in both the nucleus and cytoplasm, which is vital for shuttling between these compartments and participating in various cellular processes. BAF53′s interaction with other proteins, such as TIP49 and TIP48, is essential for mediating c-Myc oncogenic activity, highlighting BAF53′s importance in cellular growth and proliferation. Additionally, BAF53 contains a conserved N-terminal motif with specific residues at serine 2 and tyrosine 6 that are critical for activity, underscoring the intricate regulatory mechanisms that govern BAF53′s function in chromatin remodeling and gene expression.