Polyclonal Antibodies - SPAG5 Rabbit Polyclonal Antibody

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SPAG5 Rabbit Polyclonal Antibody

Catalog #：EAB11471

Datasheet

SKU-Pack Size	Availability	Size	Price
EAB11471-30UL	In Stock	30ul	￡97.90
EAB11471-100UL	In Stock	100ul	￡218.90
EAB11471-200UL	In Stock	200ul	￡372.90

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	CUlabSciences	Phone：+44 (0) 1232 330008
	Cambridge House St Thomas' Place Ely,	E-mail：sales@culabsciences.co.uk
United Kingdom	Cambridge CB27 9RD UK	Web：www.culabsciences.co.uk

Product Information
Applications	WB, ELISA
Species Reactivity	Human, Mouse
Host / Isotype	Rabbit IgG
Clonality	Polyclonal
Applications Dilutions	WB=1:500-2000
MW (kDa)	134
Conjugate	Unconjugate
Specificity	SPAG5 Rabbit Polyclonal Antibody detects endogenous levels of SPAG5 protein.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	Sperm associated antigen 5; Astrin; MAP126; DEEPEST; hMAP126; SPAG5
Gene Aliases	SPAG5
UniProt ID	Q96R06
Entrez Gene ID	10615

Product Description

SPAG5 also known as MAP126, hMAP126, DEEPEST, Astrin is a 1193 amino acid protein that is expressed at high levels in testis and at lower levels in liver, placenta, pancreas, colon and thymus. Localized specifically to spindle poles during prophase and throughout the spindle during metaphase and anaphase, SPAG5 is essential for proper spindle formation and is thought to be involved in the dynamic and functional regulation of mitotic spindles. Additionally, SPAG5 plays a role in sister chromatid cohesion and subsequent chromatid separation and, via its association with chromatids, is an important regulator of chromosome integrity. SPAG5 has a long stalk and a globular head domain through which if forms a homodimer that is characterized by aster-like structures. Upon DNA damage, SPAG5 may be phosphorylated by ATR or ATM.

Product Image Gallery
Western blot analysis of extracts on Jurkat cells, using SPAG5 Rabbit Polyclonal Antibody (EAB11471) at 1:1000 dilution. Secondary antibody Goat Anti-Rabbit IgG (H&L)-HRP (EAB21002) at 1:5000 dilution.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol