Monoclonal Antibodies - PDHA1 Mouse Monoclonal Antibody

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PDHA1 Mouse Monoclonal Antibody

Catalog #：EAB22293

Datasheet

	SKU-Pack Size	Availability	Size	Price
	EAB22293-100UL	In Stock	100ul	€297.70

If you want to get a larger packsize, Please contact your local distributor or
email us at info@ebiocell.com

Please select the country you are in to find your local distributor.

	Betaclonal	Phone：+34 (0)91 471 6763
	29/1 Lomonosovsky Pr,	E-mail：tech@betaclonal.com
Spain	Madrid, Spain	Web：www.betaclonal.com

Product Information
Applications	WB, IP, IHC-P, IF/ICC, FC, ELISA
Species Reactivity	Human, Mouse, Rat
Host / Isotype	Mouse IgG1
Clonality	Monoclonal
Applications Dilutions	WB=1:500-2000　 IP=1:100-500 IHC-P=1:100-500　 IF/ICC=1:100-500　 FC=1:50-200
MW (kDa)	43
Conjugate	Unconjugate
Specificity	PDHA1 Mouse Monoclonal Antibody detects endogenous levels of PDHA1 protein.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	PDHA1 pyruvate dehydrogenase E1 subunit alpha 1; PDHA; PDHAD; PHE1A; E1alpha; PDHCE1A; DPHA1
Gene Aliases	PDHA1
UniProt ID	P08559
Entrez Gene ID	5160

Product Description

Pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Product Image Gallery

Western blot analysis of extracts on HEK293, SKOV3, PC3, HL60, PC12 cells, using PDHA1 Mouse Monoclonal Antibody (EAB22293) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue, using PDHA1 Mouse Monoclonal Antibody (EAB22293) at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue, using PDHA1 Mouse Monoclonal Antibody (EAB22293) at 1:200 dilution.

Immunofluorescence analysis of Hela cell, using PDHA1 Mouse Monoclonal Antibody (EAB22293)(green) at 1:100 dilution.

Flow cytometric analysis of Hela cell, using PDHA1 Mouse Monoclonal Antibody (EAB22293)(green) at 1:100 dilution.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol