Monoclonal Antibodies - PDPK1 Mouse Monoclonal Antibody

Location：Home > Antibodies > Primary Antibodies > Monoclonal Antibodies

PDPK1 Mouse Monoclonal Antibody

Catalog #：EAB21893

Datasheet

	SKU-Pack Size	Availability	Size	Price
	EAB21893-100UL	In Stock	100ul	€258.70

If you want to get a larger packsize, Please contact your local distributor or
email us at info@ebiocell.com

Please select the country you are in to find your local distributor.

	SBT BioChem Srl	Phone：+39 05 7156 8147
	Via Ponte di Bagnolo, 811 Bologna,	E-mail：info@sbtbiochem.it
Italy	Italy	Web：www.sbtbiochem.it

Product Information
Applications	WB, IHC-P, IF/ICC, FC, ELISA
Species Reactivity	Human, Mouse, Rat, Monkey
Host / Isotype	Mouse IgG1
Clonality	Monoclonal
Applications Dilutions	WB=1:500-2000　 IHC-P=1:100-500　 IF/ICC=1:100-500　 FC=1:50-200
MW (kDa)	63
Conjugate	Unconjugate
Specificity	PDPK1 Mouse Monoclonal Antibody detects endogenous levels of PDPK1 protein.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	3-phosphoinositide dependent protein kinase 1; PDK1; PDPK2; PDPK2P; PRO0461; PDPK1
Gene Aliases	PDPK1
UniProt ID	O15530
Entrez Gene ID	5170

Product Description

PDPK1 (3 Phosphoinositide Dependent Protein Kinase 1) also known as PDK1 is a 556 amino acid protein that is ubiquitously expressed and localizes to the cell membrane, cytoplasm, and nucleus, playing a crucial role in various cellular processes. As a master kinase, PDPK1 is essential for phosphorylating and activating a subgroup of the AGC family of protein kinases, which are pivotal in mediating signal transduction pathways that control cell proliferation, survival, and growth, particularly in developing pancreatic beta cells. Additionally, PDPK1 regulates calcium uptake and calcium-activated potassium channels in mast cells, influences chemotaxis and motility in vascular endothelial cells, and is vital for cardiac homeostasis and thymocyte development.

Product Image Gallery

Western blot analysis of extracts on MCF7, Hela, K562, U937, A549, NIH/3T3, Jurkat cells, using PDPK1 Mouse Monoclonal Antibody (EAB21893) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.

Predicted band size: 63 kDa; Observed band size: 60-63 kDa

Immunohistochemical analysis of paraffin-embedded Human bladder cancer tissue, using PDPK1 Mouse Monoclonal Antibody (EAB21893) at 1:200 dilution.

Immunofluorescence analysis of A549 cell, using PDPK1 Mouse Monoclonal Antibody (EAB21893)(green) at 1:200 dilution.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol