Monoclonal Antibodies - MDM2 Mouse Monoclonal Antibody

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MDM2 Mouse Monoclonal Antibody

Catalog #：EAB21151

Datasheet

	SKU-Pack Size	Availability	Size	Price
	EAB21151-100UL	In Stock	100ul	€336.70

If you want to get a larger packsize, Please contact your local distributor or
email us at info@ebiocell.com

Please select the country you are in to find your local distributor.

	Cambiotech	Phone：+44 (0) 188 448801
	Lanwades Business Park,	E-mail：info@cambiotech.com
Ireland	Kobenhavn K, Ireland	Web：www.cambiotech.com

Product Information
Applications	WB, IP, IHC-P, IF/ICC, ELISA
Species Reactivity	Human, Mouse, Rat, Rabbit, Monkey
Host / Isotype	Mouse IgG2b
Clonality	Monoclonal
Applications Dilutions	WB=1:1000-5000　 IP=1:100-500 IHC-P=1:100-500　 IF/ICC=1:100-500
MW (kDa)	55
Conjugate	Unconjugate
Specificity	MDM2 Mouse Monoclonal Antibody detects endogenous levels of MDM2 protein.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	MDM2 proto-oncogene; HDMX; LSKB; hdm2; ACTFS; MDM2
Gene Aliases	MDM2
UniProt ID	Q00987
Entrez Gene ID	4193

Product Description

MDM2 also known as murine double minute 2 is an E3 ubiquitin-protein ligase. MDM2 plays a critical role in regulating the tumor suppressor protein p53 by targeting p53 for ubiquitination and proteasomal degradation. Nuclear localization of MDM2 is essential for interaction with p53, as MDM2 binds directly to p53′s transactivation domain, inhibiting transcriptional activity and promoting degradation. This regulation is crucial because p53 controls cell cycle progression and induces cell cycle arrest or apoptosis in response to DNA damage. Overexpression or amplification of MDM2 can lead to decreased p53 activity, allowing cells with damaged DNA to proliferate unchecked, which contributes to tumorigenesis. MDM2 is frequently overexpressed in various human cancers, making MDM2 an important focus in cancer research and a potential target for therapeutic intervention.

Product Image Gallery
Western blot analysis of extracts on MCF7, Mouse brain, A549 cells, using MDM2 Mouse Monoclonal Antibody (EAB21151) at 1:2000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution. Predicted band size: 55 kDa; Observed band size: 42-55 kDa

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol