Monoclonal Antibodies - AIF Mouse Monoclonal Antibody

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AIF Mouse Monoclonal Antibody

Catalog #：EAB21395

Datasheet

SKU-Pack Size	Availability	Size	Price
EAB21395-30UL	In Stock	30ul	€115.70
EAB21395-100UL	In Stock	100ul	€258.70
EAB21395-200UL	In Stock	200ul	€440.70

If you want to get a larger packsize, Please contact your local distributor or
email us at info@ebiocell.com

Please select the country you are in to find your local distributor.

	Dianost	Phone：+49 (0) 40 65472-053
	Bullenkoppel 11， 23896 Poggensee,	E-mail：ola@olasciences.com
Germany	Germany	Web：www.olasciences.com

Product Information
Applications	WB, IHC-P, IF/ICC, FC, ELISA
Species Reactivity	Human, Mouse, Rat, Monkey
Host / Isotype	Mouse IgG1
Clonality	Monoclonal
Applications Dilutions	WB=1:500-2000　 IHC-P=1:100-500　 IF/ICC=1:100-500 FC=1:50-200
MW (kDa)	67
Conjugate	Unconjugate
Specificity	AIF Mouse Monoclonal Antibody detects endogenous levels of AIF protein.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	Apoptosis inducing factor mitochondria associated 1; Apoptosis inducing factor; AIF; AUNX1; CMT2D; CMTX4; COWCK; DFNX5; NADMR; NAMSD; PDCD8; COXPD6; SEMDHL; AIFM1
Gene Aliases	AIFM1
UniProt ID	O95831
Entrez Gene ID	9131

Product Description

AIF is a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. AIF plays a crucial role in caspase-independent apoptosis. Upon apoptotic stimuli, AIF translocates from the mitochondria to the nucleus, inducing chromatin condensation and large-scale DNA fragmentation, which are key features of programmed cell death. The mitochondrial localization of AIF is important because AIF release links mitochondrial dysfunction to cell death mechanisms, contributing to various pathological conditions such as neurodegenerative diseases and cancer. Understanding AIF′s cellular location and function is significant for studying diseases where apoptosis is dysregulated.

Product Image Gallery

Western blot analysis of extracts on Hela, Jurkat, HepG2, PC12, C6 cells, using AIF Mouse Monoclonal Antibody (EAB21395) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution.

Immunohistochemistry of paraffin-embedded Human breast cancer tissue, using AIF Mouse Monoclonal Antibody (EAB21395) at 1:200 dilution.

Immunohistochemistry of paraffin-embedded Mouse kidney tissue, using AIF Mouse Monoclonal Antibody (EAB21395) at 1:200 dilution.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol