Monoclonal Antibodies - Cytokeratin 8/18 Mouse Monoclonal Antibody

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Cytokeratin 8/18 Mouse Monoclonal Antibody

Catalog #：EAB12167

Datasheet

	SKU-Pack Size	Availability	Size	Price
	EAB12167-100UL	In Stock	100ul	€258.70

If you want to get a larger packsize, Please contact your local distributor or
email us at info@ebiocell.com

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	Dianost	Phone：+49 (0) 40 65472-053
	Bullenkoppel 11， 23896 Poggensee,	E-mail：ola@olasciences.com
Germany	Germany	Web：www.olasciences.com

Product Information
Applications	WB, IHC-P, IF/ICC, ELISA
Species Reactivity	Human, Mouse, Rat
Host / Isotype	Mouse IgG2b
Clonality	Monoclonal
Applications Dilutions	WB=1:500-2000　 IHC-P=1:100-500　 IF/ICC=1:100-500
MW (kDa)	48, 54
Conjugate	Unconjugate
Specificity	Cytokeratin 8/18 Mouse Monoclonal Antibody detects endogenous levels of Cytokeratin 8/18 protein.
Purification	Affinity purification
Concentration	1mg/ml
Format	Liquid
Formulation	In PBS, pH 7.4, containing 0.02% sodium azide,0.5% BSA and 50% glycerol.
Shipping	Gel Pack
Storage	Store at -20°C least 1 year from the date of shipment. avoid repeated freeze/thaw cycles. Aliquots may be stored at +4°C for 1-2 weeks.
Research Use	For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Application Key WB-Western Blot IP-Immunoprecipitation IHC-Immunohistochemistry IHC-P-Immunohistochemistry-Paraffin IF-Immunofluorescence ICC-Immunocytochemistry FC-Flow Cytometry
Reactivity Key H-Human M-Mouse R-Rat Mk-Monkey B-Bovine Pg-Pig Hm-Hamster Dg-Dog C-Chicken X-Xenopus Z-Zebrafish Hr-Horse All-All Species Expected

Product Bioinformatics
Synonym(s)	Cytokeratin 8/18; Keratin 8; K8; KO; CK8; CK-8; CYK8; K2C8; CARD2; keratin 18; K18; CK-18; CYK18; KRT18; KRT8/KRT18
Gene Aliases	KRT8/KRT18
UniProt ID	P05787, P05783
Entrez Gene ID	3856, 3875

Product Description

Cytokeratin 8(CK8) is an intermediated filament protein with a molecular weight of 52 KD. It is a member of cytokeratin type II family. Cytokeratin 18(CK18) is an intermediated filament protein with a molecular weight of 45 KD, belongs to type I cytokeratin family. They are widely expressed in monolayer epithelial cells of thyroid , breast , gastrointestinal tract and respiratory tract. Cytokeratin 8/18 is often used as a useful marker to identify adenocarcinoma from simple epithelial cells.Cytokeratin 8/18 pair are normally expressed in simple epithelia, but not in stratified epithelial cells. Research indicates that squamous cell carcinomas derived from stratified epithelia show abnormal expression of Cytokeratin 8/18, although it is not known whether these proteins contribute to the malignant phenotype of the cells. Expression of Cytokeratin 8/18 in oral squamous cell carcinomas is an independent prognostic marker that indicates a poor prognosis.

Product Image Gallery
Western blot analysis of extracts on HCT116, SW480, HepG2 cells, using Cytokeratin 8/18 Mouse Monoclonal Antibody (EAB12167) at 1:1000 dilution. Secondary antibody Goat Anti-Mouse IgG (H&L)-HRP (EAB21001) at 1:5000 dilution. Immunohistochemical analysis of paraffin-embedded Human liver tissue, using Cytokeratin 8/18 Mouse Monoclonal Antibody (EAB12167) at 1:200 dilution.

Specific Protocols

>>	Western Blotting Protocol	>>	Immunoprecipitation Protocol
>>	Immunohistochemistry Protocol	>>	Immunofluorescence Protocol
>>	Immunocytochemistry Protocol	>>	Flow Cytometry Protocol
>>	ChIP Protocol	>>	ELISA Protocol
>>	HPLC Protocol	>>	PCR Protocol